On the 15th May, 1536, Emperor Charles V had just received news of Anne Boleyn’s arrest. Despite the treatment his aunt had received in the last years of her life due to Henry VIII, Charles was already plotting to replace Queen Anne with another of his relatives:
‘…your man George has arrived, who confirms the news touching the King’s concubine, and, as we suppose that the King will put her and her accomplices to death and take another wife, as he is of amorous complexion and always desires to have a male child, and as on the side of France they will not fail to offer him a match, you will suggest, when you can, to him or Cromwell, a marriage with the Infanta of Portugal, daughter of our sister the queen of France, who has 400,000 ducats dowry by testament. Another marriage might be arranged for the Infant Don Loys of Portugal, our brother-in-law, with the princess of England. You must point out to them that these matches would be very expedient, both to remove past scruples and to promote strict amity between us, him, and Portugal, and would be very advantageous to England in case the King should have a male child by this marriage, as he may reasonably hope from the youth and bringing up of the Infanta. If you see the King not inclined to these marriages you might propose one between the King and our niece, the duchess dowager of Milan, a beautiful young lady, well brought up and with a good dowry; treating at the same time of the other marriage between Don Loys and our cousin. But we should greatly prefer the former match with the Infanta, for the good of both, and in order to be able to dispose of our niece of Milan otherwise. Bersel, 15 May 1536.’